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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical role in endorsing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two further splice variants were being documented but specific nucleotide sequence information and facts is not but offered.

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide generally is made up of the epitope identified with the antibody.

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This tutorial outlines even more attainable optimization of cellular and immunoassay parameters to make certain the absolute best success are...

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This information outlines more probable optimization of cellular and immunoassay parameters to make certain the absolute best success are...

Antibodies bound to the blocking peptide no more bind to the epitope on the goal protein. By evaluating the staining within the blocked antibody as opposed to the antibody alone, you can see which staining is particular.

Concentrate on Facts Cyclin E belongs to your really conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which contribute for the temporal coordination of each mitotic party. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is degraded as cells development by means of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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